Coding

Part:BBa_K317998:Design

Designed by: Daisuke HIRAOKA   Group: iGEM10_Tokyo-NoKoGen   (2010-10-19)


RhlA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 720
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 720
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 69
    Illegal BamHI site found at 629
    Illegal XhoI site found at 805
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 720
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 720
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 294
    Illegal BsaI.rc site found at 478


Design Notes

The coding sequence was amplified with primer sets which were designed based on 'Standard part fabrication' described in http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication


Source

Pseudomonas aeruginosa

References